Theme: Microscopy


 

Microscopy

Electron microscopy is an unique modality for scientists attempting to map the anatomy of individual neurons and their connectivity because it has a resolution that ishigh enough to identify synaptic contacts and gap junctions. These are important indicators for types of neuron topology and are required for neural circuit reconstruction.Neural network reconstruction or connectomics is the complete mapping of all individual neurons in a region, including their synaptic contacts, to create its canonical network map, also known as a connectome.

 

theme-microscopy

 

(a) 3-D renderings of the four neurons competing for information from the muscles. The location of the synapses, which were extracted from user specified locations, are shown in red on the neurons. (b) Similar rendering of the muscles that run alongside the motor neurons.

 

Publications:

  • L. Hogrebe, A. Paiva, E. Jurrus, C. Christensen, M. Bridge, J.R. Korenberg, T. Tasdizen. “Trace Driven Registration of Neuron Confocal Microscopy Stacks,” In IEEE International Symposium on Biomedical Imaging (ISBI), 2011.
  • E. Jurrus, A.R.C. Paiva, S. Watanabe, J.R. Anderson, B.W. Jones, R.T. Whitaker, E.M. Jorgensen, R.E. Marc, T. Tasdizen. “Detection of Neuron Membranes in Electron Microscopy Images Using a Serial Neural Network Architecture,” In Medical Image Analysis, Vol. 14, No. 6, pp. 770--783. 2010.  PubMed ID: 20598935

  • T. Tasdizen, P. Koshevoy, B.C. Grimm, J.R. Anderson, B.W. Jones, C.B. Watt, R.T. Whitaker, R.E. Marc. “Automatic mosaicking and volume assembly for high-throughput serial-section transmission electron microscopy,” In Journal of Neuroscience Methods, Vol. 193, No. 1, pp. 132--144. 2010. PubMed ID: 20713087

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