Scientific Computing and Imaging Institute IEEE Institute of Electrical and Electronics Engineers

ZebraViewer

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Contents

Features

  • Paul built ZebraViewer as an extension of ITK ImageViewer with improved annotation tools:
    • allows annotation of several axons at once
    • has ability to save/load the annotations. (NOTE: you can't load the annotations saved using the ImageViewer, but you can import them).
    • can delete the annotation for a particular axon via the popup menu on the color bar - right click on a color box to bring up the menu.
    • right-click is simulated by ctrl-any-click (for one-button systems, i.e. the Mac)
    • the popup menu has options to import/export annotation points from/to a file. This can be used to load points saved via the ImageViewer.
    • you can also modify the colorbar - add/remove/insert/change colors.
    • warns you when quitting or loading a file in case you have unsaved changes.
    • implements unlimited undo/redo functionality.

Results

  • 12/2/05: Melissa has been having good luck tracking axons using this tool. Thanks Paul!

.ann file format

  • The annotation file is a simple text file with ".ann" filename extension. The file references the dataset that is being annotated, lists the maximum number of annotations (axons), and the number of points for each annotation (axon) followed by a list of points.
  • Here is the file format for .ann files (courtesy of Liz):
dataset: some_file_path
annotations: number_of_annotations (HINT: this is the same as the number of colors in the colorbar)

annotation_id number_of_points (NOTE: annotations with zero points simply haven't been tracked yet)
       x y z pixel_intensity
       ..
       ..
       ..

annotation_id number_of_points
       x y z pixel_intensity
       ..
       ..
       ..

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